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Fig. 4 | Cell & Bioscience

Fig. 4

From: Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation

Fig. 4

Production of healthy fertile offspring with in vitro-derived fSLCs. A–C The process of ICSI with fSLCs. Zygotes (D), two-cell embryos (E), four-cell embryos (F), and GFP signals in blastocysts (G, H) were observed in in vitro culture (KSOMAA medium) after ICSI with fSLCs derived from in vitro transdifferentiation. I Fluorescence was detected by live imaging under a Lumazone imaging system (MagBiosystems) and strong green fluorescence signals in offspring were shown. TG: transgenic mice; WT: wild type. J GFP sequence in the offspring detected by Southern blot analysis. TG: transgenic mice; WT: wild type. K The methylation status of the DMRs of Peg10 and H19 genes in the mother (F0) and offspring (F1), as shown by bisulfite sequencing analysis. Scale bar, 50 µm

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