Fig. 3
Induction of meiosis and formation of haploid SLCs during in vitro transdifferentiation of FGSCs. A FGSCs isolated from actin-GFP mouse ovaries were induced by Kitw/wv testicular cells, RA, and cytokines. This process was observed at different time points. B Chromosomal synapsis and different stages of meiosis (leptotene, zygotene, pachytene, diplotene) were assessed by immunofluorescence of SCP1 and SCP3. C Transcripts of meiotic factors and haploid spermatid markers were detected by RT-PCR at different time points. D The DNA content of fSLCs was determined by flow cytometry analysis. The rate of FGSCs underwent complete meiosis was the number of fSLCs at Day 14 divided by the number of FGSCs at Day 0 and then divided by 2. E Haploid cells were confirmed by karyotype analysis. F fSLCs were confirmed by ACR immunofluorescence. Scale bar, 50 µm