Fig. 2

Expression kinetics of selected genes during chondrogenesis. Expression of selected genes were quantified at different time point in 6 individual BM-MSC replicates during chondrogenesis in pellet conditions with (black line) or without TGFβ3 (hatched line) by RT-qPCR. Results are normalized to the expression at day 0 and expressed as the mean ± sem. Statistical significance of gene expression was indicated as *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001 when compared to d0 and as ^$: p ≤ 0.05, ^$$: p ≤ 0.01 when samples cultured + or − TGFβ3 were compared. (HEYL: Hes Related Family BHLH Transcription Factor with YRPW Motif Like; PDK4 (Pyruvate Dehydrogenase Kinase 4); TIPARP (TCDD Inducible Poly(ADP-Ribose) Polymerase); NDN (Necdin, MAGE Family Member); ZFP36 (ZFP36 Ring Finger Protein); TNFAIP3 (TNF Alpha Induced Protein); KCNH1 (Potassium Voltage-Gated Channel Subfamily H Member 1); FKBP5 (FKBP Prolyl Isomerase 5); PRRG4 (Proline Rich And Gla Domain;) ETS2 (ETS Proto-Oncogene 2, Transcription Factor); JARID2 (Jumonji And AT-Rich Interaction Domain Containing 2); ITPR1 (Inositol 1,4,5-Trisphosphate Receptor Type 1); SLC19A2 (Solute Carrier Family 19 Member 2); NRG1 (Neuregulin 1); PFKFB3 (6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3); SPHK1 (Sphingosine Kinase 1); NR4A1 (Nuclear Receptor Subfamily 4 Group A Member 1); PLAUR (Plasminogen Activator, Urokinase Receptor); KCNE4 (Potassium Voltage-Gated Channel Subfamily E Regulatory Subunit 4); NFKBIA (NFKB Inhibitor Alpha); TSPAN2 (Tetraspanin 2); RASD2 (RASD Family Member 2); RGCC (Regulator of Cell Cycle); NMB (Neuromedin B))