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Fig. 3 | Cell & Bioscience

Fig. 3

From: RhoA enhances osteosarcoma resistance to MPPa-PDT via the Hippo/YAP signaling pathway

Fig. 3Fig. 3

YAP alleviates the MPPa-PDT-induced apoptotic death of OS cells. a qPCR was used to assess YAP expression in lentivirally infected HOS and MG63 cells. b–e Western blotting demonstrating YAP levels in HOS and MG63 levels in lentivirally infected cells, with GAPDH as a normalization control. f, g Protein levels of YAP and its downstream targets were assessed via Western blotting, with GAPDH as a normalization control. h, i Six different human OS cell lines (HOS-shNC, HOS-shYAP, HOS-OE-YAP, MG63-shNC, MG63-shYAP, and MG63-OE-YAP) were treated with various concentrations of MPPa for 20 h and then subjected to PDT, and a CCK-8 assay was used to assess cell viability at 12 h post-MPPa-PDT. j, k CCK-8 assay results demonstrating changes in OS cell viability following YAP knockdown or overexpression at 12 h post-MPPa-PDT. l, m Hoechst staining demonstrating the apoptotic death of OS cells at 12 h post-MPPa-PDT following YAP knockdown or overexpression. n HOS and MG63 cell apoptosis rates were assessed via flow cytometry at 12 h post- MPPa-PDT treatment. o, p Western blotting analyses of protein levels at 12 h post-MPPa-PDT treatment, with GAPDH as a loading control. Experiments were repeated in triplicate. * P < 0.05 **P < 0.01 ***P < 0.001

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