Fig. 5

LOX was determined as the target of miR-30c-2-3p. A Venn diagram showing predicated target of miR-30c-2-3p by intersecting four datasets. B, C Expression of LOX in RNA level (B) and protein level (C) as indicated. D The binding sites of miR-30c-2-3p to LOX 3′UTR were mutated and engineered into a luciferase reporter vector. E Luciferase activity for each group as indicated. NS represents not significant. F Pearson correlation analysis between LOX and miR-30c-2-3p in 60 GC tissues. G qRT-PCR analysis of LOX expression after downregulating RNF144A-AS1. H Western blot analysis of LOX expression in MKN45 and AGS cells transfected with RNF144A-AS1-specific siRNA and in HGC27 cells transduced with RNF144A-AS1 vector. Abbreviation of RNF represents RNF144A-AS1. I, J Pearson correlation analysis between LOX and RNF144A-AS1 in 375 GC tissues from the TCGA database (I) and in GC cell lines (J). K Analysis of LOX expression in protein and RNA levels from treated HGC27 cells. L Detection of LOX in HGC27 cells transduced with wild-type RNF144A-AS1 expression plasmids, miR-30c-2-3p binding sites-mutated RNF144A-AS1 plasmids or control. M Quantitative analysis of RNF144A-AS1 expression as indicated. N Expression level of Dicer and LOX in GC cells treated with Dicer targeting-siRNA. O Detection of LOX in HGC27 cells co-transfected with RNF144A-AS1 vector and Dicer targeting-siRNA. Error bars, mean ± SD from triplicate samples. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t‐test unless otherwise specified