Fig. 2

RNF144A-AS1 promoted cellular migration, invasion, proliferation and angiogenesis in GC. A GSEA analysis of RNF144A-AS1 based on gene expression data from TCGA database (left panel) and CCLE database (right panel). Normalized Enrichment Score (NES) > 1, Nominal P-value < 0.05. B Expression level of RNF144A-AS1 in MKN45 and AGS cells transfected with RNF144A-AS1-specific siRNAs (left panel), and in HGC27 cells treated with lentivirus vector containing RNF144A-AS1 coding sequences (right panel). C, D Detection of the migrative and invasive ability of MKN45 (C) and AGS (D) cells transfected with RNF144A-AS1-specific siRNAs or negative control (NC) by transwell assays. Scale bars = 100 μm. E, F Detection of the tube-formation ability of HUVECs after co-cultured with conditioned medium from treated MKN45 (E) and AGS (F) cells. Scale bars = 200 μm. G, H Cell growth curves for MKN45 (G) and AGS (H) cells transfected with RNF144A-AS1-specific siRNAs or negative control. Data were analyzed using two-way ANOVA. I Cellular migration and invasion was determined by transwell assays in HGC27 cells transfected with RNF144A-AS1 vector or empty vector. Scale bars = 100 μm. J The effect of overexpressing RNF144A-AS1 on the tube-formation ability of HUVECs. Scale bars = 200 μm. Error bars, mean ± SD from triplicate samples. **P < 0.01, ***P < 0.001 by Student’s t‐test unless otherwise specified