Fig. 1

RNF144A-AS1 was upregulated in GC and predicted a poor prognosis. A Expression of RNF144A-AS1 in 375 GC tissues and 32 normal gastric tissues from the TCGA database. B qRT-PCR analysis of RNF144A-AS1 in 60 paired GC tissues. C Presentation of the percentage with upregulated RNF144A-AS1 expression in these 60 paired GC tissues. D Expression of RNF144A-AS1 in GC cells as compared with GES-1. E Expression patterns of RNF144A-AS1 in tumor tissues relative to normal tissues according to T (Tumor) stage, N (Node) stage, M (Metastasis) stage, and TNM stage from TCGA database. Comparisons were also conducted between groups for T2–3 and T1, N1–3 and N0, M1 and M0. NS, not statistically significant. F Kaplan–Meier curves showing overall survival rate and recurrence-free survival rate in GC patients stratified by RNF144A-AS1 expression from KMplotter. P-value by Log-rank test. HR Hazard ratio. G Representative images of the cellular localization of RNF144A-AS1 according to FISH assay. Scale bars = 25 μm. H RNF144A-AS1 is largely located in the cytoplasm of GC cells. Error bars, mean ± SD from triplicate samples. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t‐test unless otherwise specified