Fig. 5

Cytotoxicity of T1012G against gastric cancer cells, virus replication and expression of IFN-I response genes after T1012G infection with altered STAT3 gene expressions. A and B Cell viability (measured by cck8) of HGC-27 gastric cancer cells transfected with siRNA or plasmid was assayed 2 days after infection of T1012G at different MOI. C After 48 h of incubation with or without propranolol (40 μmol/l), cells transfected with control treatment, siRNA or plasmid were infected with different dose of virus (0.01, 0.05, 0.1, 0.5, 1 MOI). The number of surviving cells in each well was determined 2 days after infection. D, F and H Western blot analysis of STAT3 in the transfected siRNA (si-1 and si-2) and plasmid. E, G and I Cells were infected (MOI 0.01) with HSV-1 (T1012G) and virus yields were determined on Vero cells after 48-h infection. J, L and N Expression of IFN-I responsive genes, PKR, 9 or 20 h following T1012G infection (0.01 MOI) in J and L: HGC-27 cells treating with STAT3 siRNA or vehicle and N: HGC-27 cells treating with plasmid-STAT3 or vehicle. K, M and O Quantification of J, L, N. Results are presented as mean ± SEM, significant differences were evaluated using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.01 and ****P < 0.0001 (Tukey test for multiple comparisons). **P < 0.01 and ***P < 0.01 (Dunnett's test for multiple comparisons). OE overexpression