Fig. 4From: Lipocalin 2 regulates expression of MHC class I molecules in Mycobacterium tuberculosis-infected dendritic cells via ROS productionLCN2 regulates MHC class I molecule expression and CD8+ effector T-cell generation following intratracheal Mtb infection. WT and LCN2−/− mice were intratracheally infected with Mtb (106 CFU) for 10 days. A–D Expression levels of CD80, CD86, and MHC classes I and II molecules were analyzed by flow cytometry using specific antibodies in CD11c+ cell populations from mediastinal lymph nodes and lungs. Data are shown as mean ± SD (n = 18, 6 mice/group) and are pooled from three independent experiments. E, F Levels of effector T cells (CD62L−CD44+) were detected by flow cytometry in CD3+ cell populations from mediastinal lymph nodes and lungs. Data are shown as mean ± SD (n = 18, 6 mice/group) and are pooled from three independent experiments. Statistically significant differences were determined by Mann–Whitney test (non-parametric unpaired two-tailed t-test). *p < 0.05, and ***p < 0.001 LN, lymph nodeBack to article page