Table 2 Antibodies for flow cytometry, western blot and immunohistofluorescence staining
Antibody raised against | Dilution | Source (cat. NO) |
|---|---|---|
Flow cytometry antibody | ||
 PE Rat IgG2b, κIsotype Control | 1:200 (F) | BD (553989) |
 FITC Rat IgG2b, κIsotype Control | 1:200 (F) | BD (554688) |
 PE Hamster Anti-Mouse CD29 | 1:200 (F) | BD (562801) |
 PE Rat Anti-Mouse CD44 | 1:200 (F) | BD (553134) |
 FITC Rat Anti-Mouse CD34 | 1:200 (F) | BD (560238) |
 FITC Rat Anti-Mouse CD45 | 1:200 (F) | BD (553079) |
Primary antibody | ||
 Stat3 mouse mAb | 1:500 (IHF) 1:1000 (WB) | CST (9139) CST (9139) |
 P-Stat3 mouse mAb | 1:1500 (WB) | CST (4113) |
 OPN rabbit pAb | 1:200 (IHF) | ZEN BIO (380437) |
| Â | 1:1000(WB) | ZEN BIO (380437) |
 COL1A1 rabbit pAb | 1:200 (IHF) | Abclonal (A1352) |
| Â | 1:1000 (WB) | Abclonal (A1352) |
 GAPDH rabbit mAb | 1:1000 (WB) | CST (5174) |
Secondary antibody | ||
 Anti-mouse Alexafluor-594 | 1:500 (IHF) | Beyotime |
 Anti-rabbit Alexafluor-594 | 1:500 (IHF) | Beyotime |
 Anti-mouse IgG HRP-linked Ab | 1:4000 (WB) | CST (7076) |
 Anti-rabbit IgG HRP-linked Ab | 1:4000 (WB) | CST (7074) |