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Table 2 Antibodies for flow cytometry, western blot and immunohistofluorescence staining

From: Loss of signal transducer and activator of transcription 3 impaired the osteogenesis of mesenchymal progenitor cells in vivo and in vitro

Antibody raised against Dilution Source (cat. NO)
Flow cytometry antibody
 PE Rat IgG2b, κIsotype Control 1:200 (F) BD (553989)
 FITC Rat IgG2b, κIsotype Control 1:200 (F) BD (554688)
 PE Hamster Anti-Mouse CD29 1:200 (F) BD (562801)
 PE Rat Anti-Mouse CD44 1:200 (F) BD (553134)
 FITC Rat Anti-Mouse CD34 1:200 (F) BD (560238)
 FITC Rat Anti-Mouse CD45 1:200 (F) BD (553079)
Primary antibody
 Stat3 mouse mAb 1:500 (IHF)
1:1000 (WB)
CST (9139)
CST (9139)
 P-Stat3 mouse mAb 1:1500 (WB) CST (4113)
 OPN rabbit pAb 1:200 (IHF) ZEN BIO (380437)
  1:1000(WB) ZEN BIO (380437)
 COL1A1 rabbit pAb 1:200 (IHF) Abclonal (A1352)
  1:1000 (WB) Abclonal (A1352)
 GAPDH rabbit mAb 1:1000 (WB) CST (5174)
Secondary antibody
 Anti-mouse Alexafluor-594 1:500 (IHF) Beyotime
 Anti-rabbit Alexafluor-594 1:500 (IHF) Beyotime
 Anti-mouse IgG HRP-linked Ab 1:4000 (WB) CST (7076)
 Anti-rabbit IgG HRP-linked Ab 1:4000 (WB) CST (7074)