Fig. 6

The decreased ALP expression and mineralization were found on Stat3 CKO BMSCs. A The alkaline phosphatase (ALP) staining was performed at 7d of osteogenic induction. B The alizarin red staining (ARS) was done at 14d of osteogenic induction. C Quantitative data of ALP staining were obtained by ImageJ software. D Alizarin Red concentrations were determined by a quantitative destaining procedure using cetylpyridinium chloride (CPC). n = 3, **P < 0.01, ***P < 0.001 vs Con