Fig. 2

Bcl3 expression is increased during hepatocyte senescence. a Heat map of the 30 most significantly upregulated SASP or NF-κB genes in each group. b, c The expression of Bcl3 in each group was detected by IHC analysis. Bar, 100 μm. **p < 0.01 and ****p < 0.0001, as determined by t-test. d Representative image of IF costained Bcl3-positive cells (green) and HNF-4α-positive cells (red), and nuclei were stained with DAPI (blue) 5 months after HCC induction. Bar, 100 μm. e Representative image of IF stained Bcl3 (red) and p21 (green) 5 months after HCC induction. Bar, 100 μm