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Fig. 1 | Cell & Bioscience

Fig. 1

From: The hepatic senescence-associated secretory phenotype promotes hepatocarcinogenesis through Bcl3-dependent activation of macrophages

Fig. 1

The Hepatic senescence-associated secretory phenotype is evident during hepatocarcinogenesis in mice. a Schematic representation of DEN-CCl4-induced HCC mouse model. b Representative liver morphology and the number of tumor nodules in the indicated groups. c Representative image of SA-β-Gal activity (blue) in frozen liver sections and IHC stained p21 in liver sections of the indicated groups. Site of zoomed image (below). Bar: 10 μm or 100 μm. d, e The expression of SA-β-gal (d) and number of p21-positive cells (e) were evaluated in the indicated groups. f Relative p21 mRNA levels were examined by qRT-PCR and normalized to the level in the control group. g, h p21 protein expression was analyzed by Western blotting. β-actin was employed as the loading control. i IF costaining of HNF-4α−positive cells (red) and p21-positive cells (green). Nuclei stained with DAPI (blue). Bar, 100 μm. j Heat maps of the typical SASP factor expression profile as determined using the R language package. k IHC staining and quantitation of IL-8 at different stages. Bar, 100 μm. l IF costaining of p21−positive cells (green) and IL-8−positive cells (red). Nuclei stained with DAPI (blue). Bar, 100 μm. m IF costaining of HNF-4α (green) and IL-8−positive cells (red). Nuclei stained with DAPI (blue). Bar, 100 μm. The data are presented as the mean ± SD. n = 5 for each group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 between the indicated groups

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