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Fig. 3 | Cell & Bioscience

Fig. 3

From: Chorionic villus-derived mesenchymal stem cell-mediated autophagy promotes the proliferation and invasiveness of trophoblasts under hypoxia by activating the JAK2/STAT3 signalling pathway

Fig. 3

CV-MSCs promoted trophoblast growth and metastasis through the JAK2/STAT3 signalling pathway. A Cell proliferation was evaluated by a CCK-8 assay. The three trophoblast cell lines were treated with IL-6 or CV-MSC CM and with or without cryptotanshinone under hypoxic conditions. Trophoblast cells cultured alone under hypoxic conditions served as controls. B Trophoblast cells were treated with IL-6 or CV-MSC CM and with or without cryptotanshinone, and the invasion abilities were determined by Transwell assay. The number of penetrated cells was counted and analysed statistically. C The three trophoblast cell lines were cultured under hypoxic conditions with or without 3-MA or CV-MSC CM, and p-JAK2, p-STAT3, MMP2, MMP9 and LC3 expression levels were tested by western blotting analysis. D Cell proliferation was evaluated by a CCK-8 assay. The three trophoblast cell lines were treated with 3-MA or CV-MSC CM under hypoxic conditions. Trophoblast cells cultured alone under hypoxic conditions served as controls. E Trophoblast cells were treated with 3-MA or CV-MSC CM under hypoxic conditions, and invasion abilities were determined by Transwell assay. The number of penetrated cells was counted and analysed statistically. ***P < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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