Fig. 2

Global transcriptome characterization of trophoblasts regulated by CV-MSCs; JAK2/STAT3 signalling pathway activation by CV-MSCs mediates increased trophoblast autophagy. A Heat map of some differentially expressed genes in JEG-3 cells treated with or without CV-MSC CM under hypoxic conditions according to RNA-seq. B GSEA of the most upregulated and downregulated pathways in JEG-3 cells treated with CV-MSC CM compared with JEG-3 cells cultured alone. C Significant increases in STAT3, IL-6 and LIF mRNA levels were found in JAR, JEG-3 and HTR-8 cells treated with CV-MSC CM by qRT-PCR. D Significant increases in p-JAK2 and p-STAT3 were found in JAR, JEG-3 and HTR8 cells treated with CV-MSC CM under hypoxic conditions by western blotting. E Immunoblot analysis of IL-6 and LIF protein levels in normal CV-MSCs, PE-CV-MSCs and CV-MSCs under hypoxia condition. F Three trophoblast cell lines were cultured with normal CV-MSC CM or PE CV-MSC CM, and p-STAT3 expression was tested by Immunofluorescence. G Three trophoblast cell lines were cultured under hypoxic conditions with or without cryptotanshinone, IL-6 or CV-MSC CM, and JAK2, p-JAK2, STAT3, p-STAT3, LC3 and P62 expression levels were tested by western blotting analysis. The results are expressed as the means ± SD. ***P < 0.001