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Fig. 1 | Cell & Bioscience

Fig. 1

From: Chorionic villus-derived mesenchymal stem cell-mediated autophagy promotes the proliferation and invasiveness of trophoblasts under hypoxia by activating the JAK2/STAT3 signalling pathway

Fig. 1

CV-MSCs promote the autophagy, proliferation and invasion of trophoblasts in vitro. A Representative CCK-8 assay results for JAR, JEG-3 and HTR-8 cells are shown. Trophoblast cells were treated with CV-MSC CM under hypoxic conditions. B Representative Transwell assay results for trophoblasts are shown. The number of trophoblasts that migrated through the 8-μm Transwell membrane pores was counted to determine changes in the invasive capabilities in response to CV-MSC CM under hypoxic conditions (scale bar, 50 μm). C Transmission electron microscopy (TEM) analysis showed autophagosomes in the cytoplasm of trophoblast cells treated with CV-MSC CM under hypoxic conditions (scale bar, 1 μm). D Whole cell lysates from trophoblast cells were subjected to western blotting to analyse LC3, BECN1, p62, MMP2 and MMP9 levels. β-actin was included as a loading control. ***P < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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