Fig. 4From: The mechanism of Annexin A1 to modulate TRPV1 and nociception in dorsal root ganglion neuronsAnxA1 deletion sensitizes capsaicin induced TRPV1 responses. Representative traces show the injected currents induced repetitive action potentials in control (a) and AnxA1−/− (b) DRG neurons. c Quantification of action potential frequencies in control and AnxA1-deficient DRG neurons after different currents injection (20, 100, and 200 pA). (AnxA1−/− versus control group, ****P < 0.0001, Two-way ANOVA repeated measures with Tukey’s multiple comparisons test. Control group, n = 10; AnxA1−/− group, n = 8). Representative traces of d 10 μM capsaicin-gated currents, g 100 μM mustard oil-gated currents, j 100 μM menthol-gated currents and h 10 μM ATP-gated currents at -70 mV in control (black trace) and AnxA1−/− (red trace) DRG neurons. Quantification of average current density after capsaicin e (AnxA1−/− versus control group, ***P < 0.001, Student’s t-test, n = 8 neurons in WT group and n = 8 neurons in AnxA1−/− group from 3 cultures, respectively), mustard oil (h) (AnxA1−/− versus control group, P = 0.61, Student’s t-test, n = 10 neurons from 3 cultures in each group), and menthol (k) (AnxA1−/− versus control group, P = 0.94, Student’s t-test, n = 9 neurons from 3 cultures in each group), application measured at the current peak between control and AnxA1−/− group. Time constants Tau of capsaicin (f), mustard oil (i), and menthol (l)-induced activation and deactivation at -70 mV in control and AnxA1-deficient DRG neurons (AnxA1−/− versus control group, capsaicin: P = 0.81, P = 0.55; mustard oil: P = 0.79, P = 0.87; menthol: P = 0.99, P = 0.59,  Student’s t-test). All data are represented as mean ± SDBack to article page