Fig. 6

C1GALT1 is a direct target of miR-152 in GC. a Venn diagrams depicting the number of potential miRNAs targeting the 3ʹUTR of C1GALT1. b Correlation of miR-152 and C1GALT1 expression in GC tissues (Spearman’s rank correlation test). c, d qRT-PCR and Western blot analysis of C1GALT1 expression after miR-152 overexpression or inhibition. e–g Cell proliferation, migration, and invasion were assessed by CCK-8, Transwell migration, and Matrigel invasion assays. h Diagram of the potential binding sequences of miR-152 on the 3′-UTR of C1GALT1 and the mutated sequences of C1GALT1 3′-UTR. i Relative luciferase activity was examined by the dual-luciferase reporter assay. OV: cells transfected with C1GALT1 plasmid. *p < 0.05, **p < 0.01, ^#p > 0.05 compared with the mimics NC or inhibitor NC group (Student’s t-test or one-way ANOVA)