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Fig. 3 | Cell & Bioscience

Fig. 3

From: A novel mechanism for C1GALT1 in the regulation of gastric cancer progression

Fig. 3

C1GALT1 regulates the O-glycosylation of integrin α5 in GC. a The number of PNA-binding proteins identified by mass spectrometry was displayed using Venny. b The cell-surface proteins that interacted with PNA were detected using a lectin-based pull-down assay. Membrane proteins were divided into two parts, one for GADPH detection, and the other for PNA-binding measurement. GAPDH was an internal control. c The proteins from whole-cell lysates enriched by PNA were analyzed by immunoblotting with an anti-integrin α5 antibody. d The indicated protein expression was evaluated by Western blot after transfection. e Representative images of integrin α5, p-PI3K, and p-AKT staining in GC samples. Scale bars, 100 μm. f Correlation analysis (Spearman’s rank correlation test). shNC: cells transfected with negative control lentivirus; shRNA: cells transfected with C1GALT1 shRNA2 lentivirus; Mock: cells transfected with empty plasmid; OV: cells transfected with C1GALT1 plasmid; siNC: cells transfected with negative control siRNA; siRNA: cells transfected with integrin α5 siRNA; IB: immunoblot; PD: pull-down

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