Fig. 2

C1GALT1 promotes GC cell proliferation, migration, and invasion. a Bioinformatics analysis of C1GALT1 mRNA expression in 38 GC cell lines using the CCLE database. b qRT-PCR analysis of C1GALT1 expression in one normal gastric cell line and six GC cell lines. c Western blot analysis of C1GALT1 expression in different cell lines. d C1GALT1 protein expression was detected by Western blot after transfection. e C1GALT1 mRNA expression was analyzed by qRT-PCR after transfection. f CCK-8 assay was used for proliferation analysis. g Transwell chambers without Matrigel were used for migration analysis. h Matrigel-coated Transwell chambers were used for invasion analysis. shNC: cells transfected with negative control lentivirus; shRNAs: cells transfected with C1GALT1 shRNA lentivirus; Mock: cells transfected with empty plasmid; OV: cells transfected with C1GALT1 plasmid. *p < 0.05, **p < 0.01 compared with the shNC or Mock group (Student’s t-test or one-way ANOVA)