Fig. 4
AKR1B10 promotes PIP3 expression and subsequent activation of the PI3K/AKT pathway in breast cancer cells. A PIP3 ELISA kit analysis showed increased expression levels of PIP3 in MCF-7/AKR1B10 cells, and reduced expression levels of PIP3 in BT-20/shAKR1B10#1 cells, respectively; B, C Western blotting analysis showed increased protein levels of Phosphorylated-PI3K and Phosphorylated-AKT in MCF-7/AKR1B10 cells, and reduced protein levels of Phosphorylated-PI3K and Phosphorylated-AKT in BT-20/shAKR1B10#1 cells, respectively. Experiment was repeated three times. Representative results were presented. *P < 0.05