Fig. 3

AKR1B10 activates the NF-κB pathway in breast cancer cells. A, B Western blotting analysis showed increased protein levels of Phosphorylated-IKBα and Phosphorylated-NF-κB p65 in MCF-7/AKR1B10 cells, and reduced protein levels of Phosphorylated-IKBα and Phosphorylated-NF-κB p65 in BT-20 cells/shAKR1B10#1 cells, respectively; C Western blotting analysis showed decreased protein levels of nuclear NF-κB p65 in nucleus in BT-20/shAKR1B10#1 cells; D immunofluorescence colocalization experiments showed enhanced nuclear translocation of NF-κB p65 in MCF-7/AKR1B10 cells compared to vector control cells. E Immunofluorescence colocalization experiments showed decreased nuclear translocation of NF-κB p65 in BT20/shAKR1B10#1 cells compared to scramble control cells. Experiment was repeated three times. Representative results were presented. *P < 0.05; **P < 0.01