Fig. 1
AKR1B10 is highly expressed in breast cancer tissues and correlates with patient prognosis. A AKR1B10 mRNA was upregulated in cancerous breast tissue compared to normal tissue in Curtis Breast dataset; B AKR1B10 mRNA levels was greater in 8 fresh breast tumor specimens than in paired adjacent normal tissue. The AKR1B10 relative expression levels were normalized to 1 in adjacent normal tissue; C, D AKR1B10 protein levels were higher in 4 fresh breast cancer tissues than in adjacent normal tissues. Data quantification relative to β-actin levels were compared for AKR1B10. E, F The immunohistochemical staining (IHC) score of AKR1B10 in cancerous tissue was significantly higher than the scores in adjacent normal tissues. Images a–d are representative images of 4 levels of expression from 0 to 8 score, such as 0 for no staining (a), 2 for week staining (b), 4 for moderate staining (c), 6 for strong staining (d). Scale bar = 50 μm; G, H Kaplan–Meier estimation revealed significantly lower overall and disease-free survival rates in patients with AKR1B10high breast cancer than that in AKR1B10low patients; all experiments were performed in triplicate and a representative WB analysis is depicted