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Fig. 5 | Cell & Bioscience

Fig. 5

From: Influence of miR-221/222 on cardiomyocyte calcium handling and function

Fig. 5

The positive chronotropic effect of ISO is significantly reduced in miR-222-transfected neoCM. Spontaneous contraction activity was recorded at 37°C for 30 s each. ISO1 was measured 2 min after buffer change to ISO and ISO2 was measured 1.5 min after ISO1 (for exact timeline, see Methods section). A Representative contraction tracings are shown for mimic control and miR-222 for 10 s each (a.u.: arbitrary units). Corresponding movies and ROI overviews can be found in the Additional material (Additional files 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17). B Exemplary section of an ROI overview image of a neoCM monolayer (mc). Shown are three manually placed ROIs (yellow circles), scale bar: 50 µm. C While the number of spontaneous contractions was almost doubled in mc-transfected cells upon ISO application, this ISO effect is significantly reduced in miR-222-transfected cells. There is no difference between both ISO time points within each group. Data include 3 independent experiments, N  =  11 petri dishes, n  =  5–37 cells or cell clusters, depending on size of clusters or confluence of monolayer. Data are displayed as mean  ±  sem, single data points represent the mean from each petri dish, *p  <  0.05 respective to mc or corresponding control conditions as indicated

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