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Fig. 2 | Cell & Bioscience

Fig. 2

From: Influence of miR-221/222 on cardiomyocyte calcium handling and function

Fig. 2

AngII- and KCl-induced changes in [Ca2+]i are mediated by AT1R or LTCC, respectively. A–C AT1R inhibitor losartan significantly reduces the AngII-induced calcium transient in HL-1 cells. Cells were superfused either with 100 nM AngII (control) or with AngII plus 10 µM losartan. A Calcium transient peak of all cells (responding and non-responding cells combined) is reduced in losartan-treated cells. B Losartan also leads to diminished calcium transients in responding cells. C The proportion of non-responding cells is increased by losartan. Control: N  =  2, n  =  42–66 cells, losartan: N  =  2, n  =  24–39 cells (single data points). D, E LTCC blocker verapamil significantly reduces intracellular calcium increase in response to 25 mM KCl in HL-1 cells. Cells were superfused either with KCl (control) or with KCl plus verapamil. The baseline shift upon KCl superfusion is significantly reduced in verapamil-treated cells (D). F The dramatically reduced response when analysing all cells in this setting is in part due to an increase of non-responding cells. Concentration of verapamil: 20 µM, straight line indicates median, dotted line indicates mean, *p  <  0.05, N  =  4 (single data points), n  =  54–78 cells each

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