Fig. 4

miR-18b-5p acted as a tumor suppressor in breast cancer. a LncLocator predicted the subcellular localization of SNHG1. b Subcellular RNA fraction confirmed that SNHG1 was mainly located in the cytoplasm in HEK293T, MDA-MB-468, and Hs578T cells. c The intersection of TargetScan and StarBase predicted miRNAs. d, e The upregulated miRNAs after silencing of SNHG1 in MDA-MB-468 and Hs578T cells. f Expression of TERT after transfecting miR-383-5p mimics in Hs578T cells. g The expression of miR-18b-5p was downregulated after SNHG1 overexpression. h Transfection efficiency of miR-18b-5p mimics in Hs578T, MDA-MB-468, and MDA-MB-231 cells. i Colony counting kit-8 proved that miR-18b-5p inhibited cell proliferation. j–l Colony formation assay, wound healing assay and Transwell invasion assay showed that miR-18b-5p inhibited breast cancer cell colony formation, migration and invasion. Data are presented as the mean value from three independent experiments ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; *****P < 0.0001; NS no significance