Fig. 3

SNHG1 boosted TERT expression. TCGA data showed that TERT is positively correlated with SNHG1 in pan-caner (N = 9725) (a) and in breast cancer (N = 1097) (b). c GEPIA showed that TERT expression level was higher in breast cancer tissues than in normal tissues. d, e We identified that TERT gene was upregulated expressed between high SNHG1 group and low SNHG1 group with FDR = 4.45785008467688e−19, FC = 0.418132443350429 in breast cancer. f GEPIA showed that the expression of TERT a higher level in breast cancer tissue than that in normal tissue. g TERT presents a higher expression level in breast cancer cell lines than in MCF10A cell. h TERT showed higher expression level in breast cancer tissues than in paired normal tissues in our own cohort (N = 4 per group). i Transfection efficacy of siSNHG1. j Knockdown of SNHG1 downregulated TERT mRNA expression in Hs578T and MDA-MB-468 cells. Knockdown of SNHG1 also downregulated TERT protein expression in MDA-MB-468 and Hs578T cells (k). l The transfection efficiency of SNHG1 plasmid. m, n The expression of TERT RNA and protein upregulated after SNHG1 overexpression. o TERT protein had a higher expression level in the scramble group than in the sh-snhg1#3 group. p–r The tumorigenesis, tumor tissue, tumor growth and tumor weight of 4T1 cells in each group of Balb/C mice (N = 6 per group) are shown. Data are presented as the mean value from three independent experiments ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; *****P < 0.0001; NS no significance