Fig. 4

The expression of Adamts-1 in the stromal cells surrounding the implanting blastocyst during embryo implantation. a RT-PCR and real-time RT-PCR of Adamts-1 expression in the uterus during pregnancy (4–6 mice for each day of pregnancy). rPL7 represents the loading control. *P < 0.05. b, c Immunostaining of ADAMTS-1 in uterine tissues. The second panels (i to iv) show the enlarged images of the boxed areas in the first panels. White arrowheads indicate ADAMTS-1 positive cells. Three independent fields were analyzed for each mouse (3–4 mice for each day). Green and red colors indicate ADAMTS-1 and nucleus, respectively. GE glandular epithelial cells, LE luminal epithelial cells, S stromal cells, Bl Blastocyst, Em embryo, PDZ primary decidual zone. Scale Bar: 2 µm