Fig. 3
E2 regulates Adamts-1 expression via nuclear ER(s) in the uterus. a RT-PCR and real-time RT-PCR analyses of the expressions of Adamts-1 and Egr1 mRNAs in the uteri of adult OVX mice pre-treated with an ER antagonist, ICI 182,780 (500 µg/mouse) 30 min before E2 injection (200 ng/mouse) (4–5 mice in group). b Representative images of Western blot analyses examining the rapid changes in the phosphorylation status of AKT and MAPKs (ERK1/2 and p38) by E2 (3–4 mice in group). C Pharmacological inhibition of E2-dependent ADAMTS-1 and EGR1 induction by inhibitors of AKT (W; wortmannin, 14 μg/mouse), ERK 1/2 (U; U0126, 160 mg/kg), or p38 (SB; SB203580, 1 mg/mouse). Each inhibitor was administered to OVX mice 30 min before E2 administration (3–4 mice in each group). d Relative intensity of ADAMTS-1 protein expression *P < 0.05