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Table 1 List of primary and secondary antibodies used for western blotting and immunohistochemistry (IHC)

From: Axon regeneration after optic nerve injury in rats can be improved via PirB knockdown in the retina

Antibody

Source

Lot/Cat No.

Dilution

Primary antibodies

  

WB

IHC

 Goat anti-PirB

R&D, Minnesota, USA

AF2754

1:1000

1:100

 Rabbit anti-Vimentin

Abcam, Cambridge, UK

ab92547

 

1:500

 Rabbit anti-Phospho Stat3 Y705

Abcam, Cambridge, UK

ab76315

1:5000

 

 Rabbit anti-Stat3

Abcam, Cambridge, UK

Ab68153

1:2000

 

 Rabbit anti-GFP

Abcam, Cambridge, UK

Ab290

 

1:200

 Rabbit anti-GAP43

Cell Signaling, Boston, USA

#8945

 

1:200

 Rabbit anti-CNTF

Abcam, Cambridge, UK

ab175387

1:1000

 

 Rabbit anti-beta III Tubulin

Abcam, Cambridge, UK

ab18207

 

1:500

 Mouse anti-beta III Tubulin

Abcam, Cambridge, UK

ab78078

 

1:500

 Mouse anti-GAPDH

Abcam, Cambridge, UK

ab8245

1:5000

 

Secondary antibodies

    

 HRP-labeled anti-mouse IgG

Invitrogen, Poole, UK

G-21040

1:10,000

 

 HRP-labeled anti-rabbit IgG

Invitrogen, Poole, UK

31,460

1:10,000

 

 HRP-labeled anti-Donkey IgG

R&D, Minnesota, USA

HAF109

1:1000

 

 Alexa488 anti-goat IgG

Invitrogen, Poole, UK

A32814

 

1:300

 Alexa594 anti-rabbit IgG

Invitrogen, Poole, UK

A32740

 

1:300