Fig. 4
From: Axon regeneration after optic nerve injury in rats can be improved via PirB knockdown in the retina
The number of CTB-positive labeled RGCs in the retina. A Time course of treatment for each group of animals. CTB, cholera toxin subunit B; ON, optic nerve. B Schematic diagram of the detection areas used for laser confocal microscopy. The red solid circle represents the selected imaging area, and the black solid circle represents the optic nipple. C Representative photomicrographs showing the CTB-positive RGCs (red, 594) in the retina for each group. D Bar charts showing the RGC count in the retina for each group. ONI: optic nerve injury. Scale bar: 250 μm, * compared with the PBS + sham group p ˂ 0.05, ** compared with the PBS + sham group p ˂ 0.01, n = 4. ANOVA followed by a post hoc Student’s t-test were used