Fig. 1

PirB expression in the retina after ONI. A PirB (green, 488) expression in the retina after ONI obtained via immunostaining. DAPI represents nuclear staining (blue). ONI, optic nerve injury; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; RPE, retinal pigment epithelial cell layer; scale bars: 250 μm, n = 4. B The target strip and bar charts showing PirB expression in the retina tissue. GAPDH was used as an internal reference. The ratios of gray values of target proteins to those of respective internal references are provided. The experiment was repeated three times (n = 4). Data are presented as the mean ± standard deviation. One-way ANOVA was performed, and Student’s t-test was used for group-pair comparisons. *, p < 0.05