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Fig. 2 | Cell & Bioscience

Fig. 2

From: Modaline sulfate promotes Oct4 expression and maintains self-renewal and pluripotency of stem cells through JAK/STAT3 and Wnt signaling pathways

Fig. 2

The effect of the potential compounds on the expression of Oct4. A Cytotoxicity of the potential compounds against P19 cells. The P19 cells were treated with increasing concentrations of the potential compounds (2.5, 5, 10, 20 and 40 μg/mL) for 44 h. Cell viability was measured by the MTT assay. B The effect of MDLS on the expression of Oct4 at the mRNA level. P19 cells were treated with MDLS or DMSO for 24 and Oct4 mRNA levels were analyzed using RT-qPCR. C The effect of MDLS on the expression of Oct4 at the protein level. Top: P19 cells were treated with the potential compounds or DMSO for 48 h. The cells were then lysed, and the expression of Oct4 was analyzed using the western blot analysis. Equal protein loading was evaluated using GAPDH. Bottom: quantitative analysis of Western blotting data by image J soft-ware. Levels of these proteins were normalized to the corresponding GAPDH band expressed as relative fold changes in comparison to control samples. Values correspond to the mean ± S.E. of three independent experiments. D Structure of modaline sulfate (MDLS). *P < 0.05, **P < 0.01, ***P < 0.001, compared with the negative control

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