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Fig. 1 | Cell & Bioscience

Fig. 1

From: TRIM28 inhibits alternative lengthening of telomere phenotypes by protecting SETDB1 from degradation

Fig. 1

TRIM28 is preferentially located on the telomere of ALT cells and interacts with shelterin/telosome complex. A Lysates of HTC75 and U2OS cells stably expressing HAFL-TRIM28 were detected by western blot. B TRIM28 over-expressed cell lines were used in telomere ChIP assays with M2 beads, the precipitated DNA was slot-blotted and probed with a biotinylated telomere probe. IgG served as a negative control. C The data of (B) was repeated twice by independent experiments, then quantified and plotted as a histogram. *P < 0.05, Student’s t-test. D Detection of the interaction between TRIM28 and shelterin complex subunits by GST pull-down assay in 293T

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