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Fig. 4 | Cell & Bioscience

Fig. 4

From: Changes to the identity of EndoC-βH1 beta cells may be mediated by stress-induced depletion of HNRNPD

Fig. 4

siRNA knockdown of additional coding genes from transcriptome data. The endocrine hormone profiles in EndoCβH1 cells with attenuated expression of the most dysregulated coding genes seen in transdifferentiated cells are presented here. Where genes demonstrated reduced expression in somatostatin-positive cells, we have ablated their expression with siRNA technology and assayed for the appearance of somatostatin positivity in non-stressed cells. Where target genes were upregulated in transdifferentiated cells, we have ablated their expression in stressed cells, and assayed for rescue. A PHF12, (down-regulated in transdifferentiated cells), B C1ORF123, (down-regulated in transdifferentiated cells), C SENP7, (down-regulated in transdifferentiated cells), D DNAJC11, (down-regulated in transdifferentiated cells), E ZNF248, (down-regulated in transdifferentiated cells), F MALT1, (up-regulated in transdifferentiated cells), G GPN1, (up-regulated in transdifferentiated cells). Nuclei are marked with DAPI in blue, insulin (INS) is given in pink, somatostatin (SST) is given in green. Scale bars represent 0–25 μM

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