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Fig. 3 | Cell & Bioscience

Fig. 3

From: Changes to the identity of EndoC-βH1 beta cells may be mediated by stress-induced depletion of HNRNPD

Fig. 3

An siRNA against the HNRNPD gene induces the emergence of a somatostatin-positive cell population in un-stressed EndoC-βH1 clonal beta cells. A The percentage of somatostatin-positive cells that emerge in a clonal population of EndoC-βH1 cells treated with an siRNA against HNRNPD versus scrambled lipofectamine control is given on the Y axis, and the nature of treatment is given on the X axis. ***p =  < 0.0001. Error bars represent SD. B Validation of siRNA mediated HNRNPD gene knockdown by qRTPCR. The identity of the treatment (siRNA or scramble control) is given on the X axis, and the mean expression of HNRNPD is given on the Y axis. *p =  < 0.05. Error bars represent SD. C Immunofluorescence demonstrating hormone expression in EndoC-βH1 cells treated with a siRNA against HNRNPD or with scrambled transfection controls. EndoC-βH1. The top images left to right are controls without any treatment. The middle images left to right are of scramble lipofectamine controls. The bottom images left to right are of the siRNA against HNRNPD. Cell nuclei are labelled with DAPI (blue). Somatostatin (SST) expression is given in green. Insulin (INS) expression is given in magenta. Scale bars represent 0–25 μM. All statistical tests were performed using ANOVA

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