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Fig. 2 | Cell & Bioscience

Fig. 2

From: Changes to the identity of EndoC-βH1 beta cells may be mediated by stress-induced depletion of HNRNPD

Fig. 2

miR-543 and miR-181a-5p mimics and inhibitors do not induce/rescue somatostatin positivity in EndoC-βH1 cells. This figure illustrates the hormone profiles of EndoC-βH1 cells following manipulation of miR-543 or miR-181a levels and analysed using ANOVA. All data are derived from three biological replicates. A Stressed (25 mM glucose) EndoC-βH1 cells treated with miR-543 mimic. B EndoC-βH1 cells treated with 25 mM glucose only. C Non-stressed cells (in standard culture conditions) treated with an antagomiR to miR-543. D Non-stressed control (standard culture conditions) EndoC-βH1 cells. E Stressed (25 mM glucose) EndoC-βH1 cells treated with miR-181a-5p antagomiR. F EndoC-βH1 cells treated with 25 mM glucose only. G Non-stressed cells (standard culture conditions) treated with a miR-181a-5p mimic. H Non-stressed (standard culture conditions) control EndoC-βH1 cells. Cell nuclei are labelled with DAPI (blue). Somatostatin (SST) expression is given in green. Insulin (INS) expression is given in magenta. Scale bars represent 0–25 μM

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