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Fig. 1 | Cell & Bioscience

Fig. 1

From: Changes to the identity of EndoC-βH1 beta cells may be mediated by stress-induced depletion of HNRNPD

Fig. 1

Isolation of an enriched population of somatostatin-positive cells. We isolated an enriched population of somatostatin-positive cells by FACS (n = 6 biological replicates; 20,000 cells per replicate). Figure shows ungated EndoC βH1 cells that were stained and sorted by SST expression. Gating thresholds were set using unstained cells as a control and an isotype control. A Graph shows gating around the P3 FITC somatostatin-positive subpopulation. The P4 gate is surrounding those cells with no somatostatin staining. B Graph shows gating around the P3 FITC somatostatin-positive sub population. The gate was set conservatively to ensure a more pure enrichment of somatostatin-positive cells. C Graph shows log2 increase in the expression of somatostatin (SST) in the enriched population of somatostatin-positive beta cells. D Graph shows log2 decrease in the expression of insulin (INS) in the enriched population of somatostatin-positive beta cells. **p =  < 0.01

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