Fig. 6

Western blot analysis of LDHA and total LDH throughout chick lung branching. Representative immunoblots for a LDHA and c LDHT (LDHA and LDHB contribution) of pooled-tissue samples of embryonic lungs at D0 (0 h) (b1, b2, b3) and D2 (48 h) (B1, B2, B3) of explant culture. Loading control was performed using β-tubulin (55 KDa). LDHA and LDHT correspond to 37 KDa. Semiquantitative analysis, of three independent experiments, for b LDHA and for d LDHT protein expression levels. Results are represented in arbitrary units, normalized for β-tubulin. Results are expressed as mean ± SEM (n = 3/stage/condition). One-Way ANOVA and Fisher’s LSD test were performed. Significantly different results are indicated as: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Full-length blots are presented in Additional file 1: Figure S2