Fig. 5

Proliferation analysis of lung branching morphogenesis. Representative confocal microscopy fluorescence images of lungs explants after 48 h in culture (B1, B2, B3). a, d, g Nuclei were stained with Hoechst 33342 (Red). b, e, h Proliferation was assessed using an EdU-based assay; EdU incorporation in DNA was detected using Alexa Fluor 488 (Green). c, f, i Overlay represents the merged images of Hoechst (Red) and EdU (Green). All images are represented as maximum intensity projection of z-stacks. n ≥ 4 per stage. Scale bar: 500 μm. Asterisk: secondary bronchi/active branching sites. White arrow: distal tip. White arrowhead: trachea region