Fig. 3

mRNA expression levels of glucose catabolism enzymes in early stages of lung branching. Relative expression levels of a hk1, b pfk1, c ldha, d ldhb, e pdha, and f pdhb, in embryonic lungs at D0 (0 h) (b1, b2, b3) and D2 (48 h) (B1, B2, B3) of explant culture. g Expression fold variation, from 0 to 48 h of explant culture, for hk1, pfk1, ldha, ldhb, pdha, and pdhb. mRNA expression levels were normalized for both 18 s and actin-β housekeeping genes. Results are expressed in arbitrary units, as mean ± SEM (n ≥ 5/stage/condition). One-Way ANOVA and Fisher’s LSD test were performed. Significantly different results are indicated as: *p < 0.05; **p < 0.01; ***p < 0.001