Fig. 6
From: Exosomal delivery of therapeutic modulators through the blood–brain barrier; promise and pitfalls
(Adapted with permission [72]. Copyright 2019, ACS Nano)
Recruiting recycling endosomes, abluminal SNAREs, and Caveolin-independent pathway by transcytosis-depending of Br-EVs through BBB. a Flow cytometry analysis of BBB crossing of Br-conjugated EVs under the administration of different inhibitors of endocytosis pathway compared to the control groups. b Fluorescence images exhibiting TdTom-Br-EVs co-localization with dextran and Alexa transferrin, markers of micropinocytosis and clathrin-dependent endocytosis, subsequently. The co-localization of Br-EVs with Rab11 (c) DQ-Ovalbumin (d) Co-localization of Rab11 and DQ-Ovalbumin positive Br-EVs (e) VAMP3 (f) VAMP7 (g) Co-localization of VAMP-3 and VAMP7 positive Be-EVs (h) Syntaxin (i) and Snap3 (j). The white arrows represent co-localization