Fig. 2
From: Pathological alterations in the gastrointestinal tract of a porcine model of DMD
Generation and identification of DMD exon 51 defective pigs. A The construction flowchart of Bama miniature pigs with edited DMD exon 51. ①② Isolation and culture of miniature PFFs; ③ Transfection of miniature PFFs with Cas9/sgRNA; ④ Single cell clone picking and culturing; ⑤ Acquisition of oocytes; ⑥ Enucleation of oocytes; ⑦ Somatic cell nuclear transfer; ⑧ Embryo transfer; ⑨ Delivery and identification of DMD-delE51 pigs. B PCR analysis of DMD exon 51 in all piglets. C Mutation analysis by T-cloning and Sanger sequencing in all piglets. WT sequence is shown at the top. PAM sites are highlighted in red; target sequences are shown in green. D The relative expression of DMD mRNA in DMD-delE51 pigs and the age-matched wild-type pigs. DIA diaphragm, GAST gastrocnemius; ***P < 0.001. E Western blotting showed the disrupted expression of dystrophin in DMD-delE51 pigs. F IHC analysis of dystrophin expression in heart, diaphragm and gastrocnemius muscles. Scale bars: 50 µm and 100 µm