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Fig. 1 | Cell & Bioscience

Fig. 1

From: Pathological alterations in the gastrointestinal tract of a porcine model of DMD

Fig. 1

PFFs with DMD exon 51 deficiency showed impaired cell membrane integrity and early cell apoptosis. A Sanger sequencing of PFFs showed different mutations induced by Cas9/sgRNA electrotransfection. WT sequence is shown at the top of the targeting sequence. PAM sequences are highlighted in red. B NRD uptake assay of PFF clones carrying DMD exon 51 mutations at different cell densities. ***P < 0.001, **P < 0.01 and *P < 0.05. C LDH activities in culture medium at different time points were measured by the LDH-kit. ***P < 0.001, **P < 0.01 and *P < 0.05. D Cell apoptosis was analyzed by flow cytometry. *P < 0.05. E Western blotting analysis of autophagy in PFFs did not detect significant difference between WT and DMD-mutant PFFs

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