Fig. 5

Effect of ANT1 supplement on MPP⁺-treated neuroblastoma SH-SY5Y cells. a The map of pcDNA3.1-ANT1 which was transfected into the MPP⁺-treated neuroblastoma SH-SY5Y cells and overexpressed ANT1 protein. b Analysis of ANT1 abundance in various cell models detected by Western blot. Cells lysates containing 80 μg soluble proteins were subjected to Western blot analysis coupled with 15% SDS-PAGE separation. The membrane was probed with monoclonal anti-ANT1 antibody and visualized using a ECL kit. M, Page Ruler prestained protein ladder (Fermentas). c Densitometric analysis of ANT1 normalized to the level of GAPDH. The analyses were conducted by a two-tailed equal variance Student’s t test. Bars represented the mean  ±  SEM; *P  <  0.05. d Analysis of TH abundance in various cell models detected by Western blot analysis. Cells lysates containing 60 μg soluble proteins were subjected to Western blot analysis coupled with 10% SDS-PAGE separation. The membrane was probed with monoclonal anti-TH antibody and visualized using a ECL kit. M, Page Ruler prestained protein ladder (Fermentas). e Densitometric analysis of TH normalized to the level of GAPDH. The analyses were conducted by a two-tailed equal variance Student’s t test. Bars represented the mean  ±  SEM; *P  <  0.05; **P  <  0.01. f Densitometric analysis of DA levels detected by HPLC. g Apoptosis analysis of neuroblastoma cells using AnnexinV-FITC/PI staining and flow cytometry