Fig. 3

N interacts with Nsp3 through its NTD. A Schematic diagram of SARS-CoV-2 N protein structure. HEK293T cells were transfected with the indicated combinations of plasmids. The cells subjected to co-immunoprecipitation with Flag agarose (B) and immunostaining with Flag and HA antibodies (D–F). C The intensities of HA or Flag stained bands of each sample were quantified using LI-COR Image Studio software, and ratios of the intensities of HA/Flag bands were calculated. Note that compared with N, NTD interacted with Nsp3.1 in a stronger manner. Similarly, the interaction between Nsp3.1 and SR of N was examined using co-immunoprecipitation with GFP antibody (G). Their interactions were quantified by calculating the ratios of intensities of HA/EGFP bands (H). Note that SR of N lost the capacity to interact with Nsp3.1. Data represent one of 3 independent experiments with similar results; error bar represent mean ± s.e.m; *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed unpaired Student’s t-test