Fig. 1

Protein interactions of SARS-CoV-2 detected using mammalian two-hybrid assays. A A representative result of positive interaction. The combination of pM-53 and pVP16-T was used as the positive control. B Interaction matrix of SARS-CoV-2 proteins. Black squares indicate the interactions reported previously, and blue squares indicate the novel interactions detected in this study. C The interactions were analyzed with Cytoscape. The darker blue circles indicated that Nsp1 and Nsp10 had more interacting partners than the blue circles-labeled proteins. The red circles indicate that the proteins had self-interactions. The sticks linked different circles depicted the interactions, and the thickness of the sticks was correlated with the strength of interactions, which was judged arbitrarily based on the results of mammalian two-hybrid assays. Data represent one of 3 independent experiments with similar results; error bar represent mean ± s.e.m; *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed unpaired Student’s t-test