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Fig. 5 | Cell & Bioscience

Fig. 5

From: SARS-CoV-2: from its discovery to genome structure, transcription, and replication

Fig. 5

The expression of sgRNAs during human coronavirus infection. On the left are the diagrams of SARS-CoV-2 (A), hCoV-OC43 (B) and hCoV-NL63 (C) genomes and their coding potentials. Individual sgRNAs (lines) with a 5′ leader (small red box) obtained through the template switch are illustrated below and named by their corresponding proteins encoded. The viral gRNA (vgRNA) is generated by continuous transcription of the entire viral genome. On the right are the sgRNA expression profiles in African monkey kidney Vero E6 cells infected for 24 h with SARS-CoV-2 (A), 189 h with hCoV-NL63 (C), or human colorectal adenocarcinoma HCT-8 cells infected for 48 h with hCoV-OC43 (B). The sgRNAs detected by Northern blot analysis of total RNA extracted from infected cells using the individual antisense probes specific to each viral N gene. The Northern blot gel of SARS-CoV-2 sgRNA in A was modified with permission from a reference [38]

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