Fig. 3
One-dimensional 1H HR-MAS NMR spectra and PLS-DA analysis of intact lepb mutant zebrafish larvae. A The representative spectra of intact larvae from wild type and lepb mutant groups measured by HR-MAS NMR spectrometry. Spectra from chemical shift 0.5–4.4 were assigned to specific metabolites. Acet acetate, Ala alanine, Asp aspartate, Cho choline, Chol cholesterol, Cit citrulline, Cys cysteine, Eta ethanolamine, FA fatty acid, Glc glucose, Gln glutamine, Glu glutamate, Gly glycine, His histidine, Ile isoleucine, Lac lactate, Leu leucine, Lys lysine, Met methionine, m-Ins myo-inositol, Pu putrescine, Ser serine, Tau taurine, tCr total creatine (creatine + phosphocreatine), Thr threonine, TAMO trimethylamine N-oxide, HR-MAS NMR high-resolution magic-angle-spinning nuclear magnetic resonance. B PLS-DA analysis of intact larvae from wild type and lepb mutant groups, n = 3, three times measurements, each replicate represents 120 pooled larvae. PLS-DA partial least square discriminant analysis. C A Venn diagram is shown of the common 25 metabolites that are significantly changed both in extracted zebrafish larvae measured by 1H solution NMR and intact larvae measured by 1H HR-MAS NMR