Fig. 3

PIAS1 synergizes with SAMHD1 to restrict EBV replication. A The schematic representation of full length PIAS1 (1–651) or PIAS1 truncation mutants. B The central and C-terminal part of PIAS1 interacts with SAMHD1. 293 T cells were co-transfected with SAMHD1 and V5-tagged full length PIAS1 (aa 1–651) or PIAS1 truncation mutants as indicated. WB analyses showing that SAMHD1 is Co-IPed with the central and C-terminal part of PIAS1. Co-IP, co-immunoprecipitation; β-actin blot was included as loading controls. Arrow denotes modified SAMHD1 in the PIAS1 and SAMHD1 co-transfection group. C Full-length PIAS1 synergizes with SAMHD1 in restricting EBV replication. HEK293 (EBV +) cells were co-transfected with plasmid DNA encoding ZTA (lytic trigger), SAMHD1, and full-length (WT), truncated PIAS1 (1–205, 1–415, 1–433 and 409–651) or E3 ligase-deficient (C351S) mutant as indicated. The relative EBV copy numbers were measured using the qPCR as described in the method. The expression levels of SAMHD1 and PIAS1 were monitored by WB. β-actin blot was included as loading controls. Results from three biological replicates are presented. Error bars indicate the standard deviation. * p < 0.05, ** p < 0.01